Aktivitas antioksidan krim kombinasi ekstrak Eucheuma Cottonii Sumbawa dan ekstrak Citrus lemon L. impor dengan metode DPPH

Dwi Kurniawati Sambodo, Nandia Arlesia

Abstract


Cerrenty, The high exposure for free radicals has a negative impact on the health and aesthetics of the skin, especially facial skin, because it requires cosmetic that contain antioxidants to counteract and reduce the negative effects of it. Eucheuma cottonii and Citrus lemon L have bioactive compounds that have the potential as antioxidants. This research was conducted to find out antioxidant activity and the most optimal formula of the three cream formulas by DPPH method. Eucheuma cottonii and Citrus lemon L peel were extracted by remaseration method. The cream is formulated in 3 formulas with a ratio of extracts of 1: 2, 2: 1, and 1: 1 in 100 grams of cream with positive control of vitamin C. The three formulas were tested qualitatively and quantitatively by DPPH method with spectrophotometry visible at maximum wavelength 517 nm uses IC50 value. Qualitative test shown that the three cream formulas had antioxidant activity by DPPH damping which were characterized by reduced intensity of purple color. Quantitative test results in IC50 value formula 1 was 11251.5 ppm, formula 2 was 8592.2 ppm, and 10025.5 ppm for formula 3. From too  concluded that the three creams have antioxidant activity and formula 2 has the most optimal antioxidant than 2 other formulas


Keywords


Antioxidant activities; Eucheuma cottonii; Citrus lemon L Peel; DPPH

Full Text:

PDF

References


Winarsih H. Antioksidan Alami & Radikal Bebas Potensi dan Aplikasinya dalam Kesehatan. Yogyakarta: Kanisius. 2007;

Sharon N, Anam S, Yuliet. Formulasi Krim Antioksidan Ekstrak Etanol Bawang Hutan (Eleutherine palmifolia L. Merr.). Online J Nat Sci. 2013;

Muchtadi D. Antioksidan dan Kiat Sehat di Usia Produktif. Alfabeta. Bandung: Alfabeta; 2013.

Maharany, F., Nurjanah, R. Suwandi, E. Anwar dan TH. Kandungan senyawa bioaktif rumput laut Padina australis dan Eucheuma cottonii sebagai bahan baku krim tabir surya. JPHPI. 2017;

Luning. Seaweeds: Their Environment, Biogeography and Ecophysiology. New York John Willey Sons,Inc. 1990;

Nizhar U. Level Optimum Sari Sebagai, Buah Lemon (Citrus limon) Pembentukan, Bahan Penggumpal pada Program, Curd Keju Cottage. 2012.

Kumalaningsih S. Antioksidan Alamai Penangkal Radikal Bebas. Trubus Agisarana. 2006;

Prakash. Antioxidant Activity. Medallion Lab Anal Prog. 2011;

Luvita Gabriel Zulkarya EDH. Formulasi Sediaan Krim Ekstrak Etanol Rumput Laut Coklat (Padina Australis) Dan Uji Aktifitas Antioksidan Menggunakan Metode DPPH. Cendekia J Pharm. 2018;2 No. 1(E-ISSN 2599 – 2155).

Krisnawan AH, Budiono R, Sari DR, Salim W. Potensi Antioksidan Ekstrak Kulit Dan Perasan Daging Buah Lemon (Citrus Lemon) Lokal Dan Impor. Pros SEMNASTAN. 2018;

Umar I. Formulasi Dan Uji Efektifitas Antioksidan Ekstrak Etanol Daun BOTTO’ - BOTTO’ ( Chromolaena odorata L.) Dengan Metode DPPH. Makasar; 2014.

Blois MS. Antioxidant determinations by the use of a stable free radical [10]. Nature. 1958.




DOI: https://doi.org/10.32504/hspj.v3i1.95

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Web
Analytics View My Stats

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.